[1] The museum collects antique locomotives and rolling stock to run on their 7 miles of track from the K-25 facility in Oak Ridge, Tennessee to an interchange with Norfolk Southern at Blair, Tennessee and back.

The museum formerly ran an excursion train along this route using their rolling stock.

The museum's ticket office is an old guard station that has been restored.

In 2000, the reuse committee of the Community Reuse Organization of East Tennessee approved a lease of land to the museum for a visitor center, train depot and repair shop.

[3] In 2016 the museum ceased passenger excursion train operations but continues the restoration and preservation missions while seeking out a new home for excursions.