For many years, from the turn of the 16th-17th century, it was gradually built by anonymous people, then by Carlo Lurago and Francesco Caratti.

As early as 1578 - 1581, the Society of Jesus, led by Rector Giovanni Paolo Campana, laid the foundations of the building, then a chancel and a transept were built.

In 1581, the primate of the neighboring Jewish ghetto, Mordechai Maisel donated 100 thalers to build the church, which testifies to a completely unprecedented tolerant atmosphere and the peaceful coexistence of different religions within Rudolfinian Prague.

[5] The adjacent dormitory included the missionary Karel Slavíček, the later Bishop Franz von Dietrichstein, Tomáš Pešina of Čechorod, Saint Jan Sarkander, and the revivalist Josef Dobrovský.

[6]  After the Velvet Revolution, priests Aleš Opatrný (1990–1991), Jan Jandourek (1993–1995) and Milan Norbert Badal OP (1995–1996) worked in the church, among others.