Ambulance Victoria provides emergency medical response to more than 5.9 million people in an area of more than 227,000 square kilometres.

On 22 April 2008, Premier John Brumby and Health Minister Daniel Andrews announced a record funding boost of over $185m, including two new helicopter services, 26 new ambulance stations and over 300 new paramedics.

Many emergency services vehicles, including ambulances, are fitted with mobile data terminals that enable them to view information, read messages sent by call-takers and dispatchers, and be notified of updates immediately as they become available.

A number of communication services used by Ambulance Victoria, such as digital radio and mobile data terminals, are not available outside metropolitan Melbourne.

AV also operates Mobile Intensive Care Ambulances which are staffed by more specially trained paramedics and different equipment.

AAV operates five twin engine AgustaWestland AW139 helicopters provided by Babcock Mission Critical Services Australasia.

[38][39] In January 2017, the final of the five new AgustaWestland AW139s entered service replacing the Victoria Police Eurocopter AS365 Dauphin and ending the partnership.

[40] The aircraft are staffed by MICA Flight Paramedics who are trained to administer blood, conduct ultrasounds, complete complex procedures and perform winch rescues.

[41][42][9] AAV has operated four Beechcraft Super King Air B200s, provided by Pel-Air Aviation, from its Essendon headquarters since 2011 and can reach most of Victoria within an hour.

This service includes bringing people to Melbourne for regular treatments such as oncology and dialysis while also facilitating acute medical conditions requiring surgery or the transfer of injured patients from rural hospitals to specialist care.

[42] On 23 March 2016, the Victorian Auditor-General tabled a damning report into "Bullying and Harassment in the Health Sector".

[47] Part of Mr Lay's response included "achieve[ing] sustainable culture change within Ambulance Victoria.

"[47] On 26 October 2020, Allegations of widespread bullying, harassment and gross misconduct were public reported by The Age after 13 Paramedics raised an open letter to Mr Ken Lay, chairman of the board of directors.

[49] The Age noted that: On 27 October 2020, the Victorian Equal Opportunity and Human Rights Commission announced that it had been asked to conduct a review into allegations of unlawful, discriminatory behaviour at Ambulance Victoria by.

On 29 November 2021, almost a one-year after the allegations were first publicly raised, a number of Ambulance Victoria Employees, had reported that "in some cases victimisation has worsened since the inquiry began.