[7][8][9][10] First published in 1984, the comic continues to this day, with Sakai as the lone author and nearly sole artist (Tom Luth serves as the main colorist on the series, and Sergio Aragonés has made two small contributions to the series: the story "Broken Ritual" is based on an idea by Aragonés, and he served as a guest inker for the black-and-white version of the story "Return to Adachi Plain" that is featured in the Volume 11 trade paperback edition of Usagi Yojimbo).

The Japanese American National Museum in Los Angeles's Little Tokyo presented an exhibit entitled "Year of the Rabbit: Stan Sakai's Usagi Yojimbo" from July 9 through October 30, 2011.

The seventh episode of the 2012 Teenage Mutant Ninja Turtles animated series' fifth season, "Yojimbo", was written by Sakai and features Miyamoto Usagi, who has appeared in a few TMNT shows to date.

[12] In April 2022, Sakai was reported among the more than three dozen comics creators who contributed to Operation USA's benefit anthology book, Comics for Ukraine: Sunflower Seeds, a project spearheaded by IDW Publishing Special Projects Editor Scott Dunbier, whose profits would be donated to relief efforts for Ukrainian refugees resulting from the February 2022 Russian invasion of Ukraine.

[13][14] Sakai's contribution is an original Usagi Yojimbo story in which the main character come to the aid of refugees fleeing the invasion of their land by a warlord, themes that speak to the events in Ukraine.