[1] Lane Poole was Born in London, England, the eldest of three children (two sons and a daughter) of Edward Stanley Poole (1830–1867) and his wife, Roberta Elizabeth Louisa (1828–1866), daughter of Charles Reddelien, a naturalized German.
His paternal grandmother Sophia Lane Poole, uncle Reginald Stuart Poole and great-uncle Edward William Lane were famous for their work in orientalism and archaeology.
His other great-uncle was Richard James Lane, a distinguished Victorian lithographer and engraver.
[2][3] From 1874 to 1892 he worked in the British Museum, and after that in Egypt researching on Egyptian archaeology.
From 1897 to 1904 he had a chair as Professor of Arabic studies at Trinity College Dublin.