Born in Catania (Sicily), Stefania Spampinato began studying dance at the age of six.

In 2011, Spampinato moved to Los Angeles, where she continued to study acting.

[4] After taking part in TV series such as Glee and Satisfaction, Spampinato joined the cast of Grey's Anatomy in 2017 as Dr. Carina DeLuca,[4][5] sister of Dr. Andrew DeLuca and wife of Lt. Maya Bishop.

From 2020 to 2024, she continued to play the same role in the TV series Station 19, the second spin-off from Grey's Anatomy.

[6] In 2019, she was chosen as the co-star of the comedy The Most Beautiful Day in the World, interpreted and directed by Alessandro Siani.