[3] The web has a diameter of about 30 cm and is attached to a retreat made out of silk and covered with debris and food remains.

[4] A form of courtship behaviour shown by males of this species is creating vibrations on the female's webs before mating.

It is suggested that females may create a web-borne pheromone that causes males to display this web vibrating behavior.

Polyandry in Stegodyphys lineatus avoids inbreeding and reduces the genetic incompatibilities with matings of related individual.

[10] Egg sacs are lost quite frequently due to predation by ants and for this reason females are able to lay another clutch if they lose their first.

Males go about this by detaching the egg sac with their chelicerae, moving it to the entrance, then simply tossing it to the ground.

[14] The desert spider shows low rates of polygamy,[7] and sexual selection and environmental cues play an important role during mate search and reproductive success.

Mature females release a pheromone to reduce their attractiveness towards males due to the high costs of re-mating for the egg sac (infanticide mentioned above).

However, a high chance of multiple mates can increase the likelihood of genetic compatibility in embryo formation.

It is important to note that although multiple mates can increase the likelihood of genetic compatibility, it cannot be considered a major fitness advantage.

Migration promotes genetic variation due to decreasing the rates of inbreeding through the introduction of males from other nests.

Instances of migration that influence genetic variation can be observed through: natal philopatry (the tendency for an organism to stay near or return to where it was born) and founder events (when a small subset of a population founds a new population in a different location) lead to the survival of some individuals and the extinction of others.

More over, when siblings, males or females disperse to nearby nests, random mating occurs, which will enhance the differentiation among spiders in the following generation.

Examples of this behaviour include re-mating, offspring produced by multiple fathers and high rates of male infanticide.

Nonrandom mating can occur in this species within sibling groups because juveniles do not travel very far from the vicinity of where they were born.

[19] Female dispersal to new locations has shown to have a larger impact on enhancing genetic differences amongst offspring than can be overcome by males moving between the populations.

[19] The environment of spiders should be taken into consideration when studying the structure of a population, especially with S. lineatus that can reside in both stable and unstable conditions.