He then wrote several titles for Fantaco in the early 1990s, including Bad Moon, Fly in My Eye, and a set of lithographs for Clive Barker's Book of Blood.

When IDW Publishing formed, Niles sent his "reject list" to Ted Adams, who selected 30 Days of Night as a concept he was interested in.

[2] In 2004 or 2005, Niles formed a joint venture production company, Creep International, with 1990s rocker and film director Rob Zombie.

This project has produced two works to date: The Nail (with Nat Jones) through Dark Horse Comics, and Bigfoot (with Richard Corben) through IDW Publishing.

Upon placing that venture on hiatus, he then assisted actor Thomas Jane's production company, with the intention of bringing his Criminal Macabre character Cal MacDonald to the big screen.

[2] In 2006, Niles collaborated with artist Scott Hampton on a Batman miniseries, Gotham County Line, published by DC Comics.

That same year, he wrote another miniseries that reinvents Steve Ditko's vintage character The Creeper with DC artist Justiniano.

Dark is a vigilante hero with elements of the Frankenstein mythos[4] who starred in eighteen issues of his own ongoing series.

Narrated by Poison Ivy, the story tells of Superman, Lois Lane, and Jimmy Olsen uncovering zombies in a cargo tank owned by Lex Luthor.

[10] An X-Files/30 Days of Night crossover in 2010 was co-written by Niles and Adam Jones, the guitarist for the band Tool, with art by Tom Mandrake.

[16] In March 2012, Niles joined Halo-8 president/Godkiller writer-creator Matt Pizzolo and Epitaph Records owner/Bad Religion guitarist-songwriter Brett Gurewitz to form Black Mask Studios with the mandate of developing new ways to support creators and reach broader audiences beyond fandom.

[22] On September 28, 2018 it was announced that Niles' comic, The October Faction would be adapted into a TV show on Netflix with a first season consisting of 10 episodes.

[25] Other comics he has written that have been optioned for film include, Criminal Macabre, In the Blood, Aleister Arcane and Wake the Dead.

[32] In June 2016, Variety reported that Jim Carrey will star in Eli Roth's film adaptation of Aleister Arcane for Amblin Entertainment.