The men's 50 metre butterfly at the 2007 World Aquatics Championships took place on 25 March (heats, a swim-off, and semifinals) and on the evening of 26 March (final) at Rod Laver Arena in Melbourne, Australia.

Existing records at the start of the event were: A swim-off for 8th place should have occurred between Jakob Andkjær and Lyndon Ferns who tied for 8th at 23.99; however, results from a semifinal swim-off in the event are not on the OmegaTiming site, nor in the "complete" result page.

Andkjær did advance to the finals, as he swam there, and Ferns did not.

Following the conclusion of the preliminary heats there was a three-way tie in for 16th at 24.06 between Germany's Thomas Rupprath, and Slovenia's two entries in the event: Peter Mankoč and Jernej Godec.

As France's Frédérick Bousquet scratched the final, a swim-off to the swim-off tie was not need, and both Godec and Mankoč advanced to the semifinals.