The women's 400m freestyle S13 event at the 2008 Summer Paralympics took place at the Beijing National Aquatics Center on 8 September.

There were two heats; the swimmers with the eight fastest times advanced to the final.

The final produced a dead heat for third place and two bronze medals were awarded.

The silver medal was won by Anna Efimenko of Russia, a more impaired S12 competitor.

The gold medal was won by Valerie Grand Maison who set a world-record of 4:28.64 in the final.