The women's 100 metre breaststroke competition at the 2014 South American Games took place on March 10 at the Estadio Nacional.

[1][2] The last champion was Agustina de Giovanni of Argentina.

[4] All times are in minutes and seconds.

The first round was held on March 10, at 12:19.

[1] The final was held on March 10, at 20:42.