Synthetic genetic array

[1] SGA allows for the systematic construction of double mutants using a combination of recombinant genetic techniques, mating and selection steps.

[6][7] Synthetic genetic array analysis was initially developed by Tong et al.[1] in 2001 and has since been used by many groups working in a wide range of biomedical fields.

The double mutants are screened for SSL interactions visually or using imaging software by assessing the size of the resulting colonies.

Due to the large number of precise replication steps in SGA analysis, robots are widely used to perform the colony manipulations.

Computer programs can be used to analyze the colony sizes from images of the plates thus automating the SGA scoring and chemical-genetics profiling.

Arrayed yeast showing synthetic lethal interactions. Synthetic lethal interactions are those pairs of colonies with reduced or no growth.
Replicating yeast colonies during SGA analysis using a pinning robot