The primary set of people movers are automated guideway transit (AGT) systems that connect the airport's main terminal to four satellite airside concourses.

A fifth people mover line known as SkyConnect, which began operating in 2018, connects the main terminal with the airport's economy parking garage and rental car center.

In addition, a monorail once connected the main terminal and the long-term parking garage from 1991 until its closure in 2020.

[3] Tampa Mayor Jane Castor delivers pre-recorded greetings to travelers on the shuttles as they run between the stations.

[5] When the terminal opened in 1971, the landside/airside shuttles were the first automated people mover systems to operate in an airport.

As part of the renovation, the shuttle stations were rebuilt and moved outward to give the center of the building more space on Level 3.

The Airside D shuttle station, which had been simply blocked off with a shoe shine stand since its closure, was also removed at the same time and was replaced with a food court.

Alstom, which acquired Bombardier's transportation division in 2021, will supply the new Innovia APM 300R trains which will debut in 2025.

[17] An additional people mover line named SkyConnect connects the main terminal with the rental car center and the economy parking garage.

The guideway primarily runs along the airport's entrance road, George J. Bean Parkway, and passes underneath Taxiway J to connect with rental car and parking facilities.

[19] An automated monorail system also once connected the main terminal on Level 5 to the long-term parking garage,[20] stopping at each elevator bank in both buildings.

In the short-term garage, it ran in a straight path between the four elevator banks, which passengers could use to access the terminal.

Thales Rail Signalling Solutions won a contract in 2008 to completely upgrade the computer control system.

[21] Following the monorail's closure in early 2020, the airport opted not to invest in a replacement system due to high costs.