The Tashkent Teacher's Seminary (Russian: Ташкентская учительская семинария) was a seminary in Emir Timur Square, Tashkent, established in 1879 to train teachers for local schools, including native Russian schools.

Since 1884, with the efforts of Vladimir Nalivkin, the seminary began to teach the Uzbek language, which replaced Kazakh.

Initially, the seminary was located in the house of Colonel Tartakovsky, rebuilt according to the plans of Alexei Benois from 1881 to 1887.

Under the Soviet Union, the building housed the City Health Department of the Tashkent Executive Committee.

In 1898, E. P. Dubrovin, according to a plan drawn out by Alexei Benois, built a five-domed church in the name of Saint Alexander Nevsky in the Western part of the seminary building.