Polo's acting debut, in 1987, was in the role of Kristin Larsen on the ABC daytime soap opera Loving.

In 2000, Polo became well known for her role as Pam Byrnes in the comedy Meet the Parents, opposite Ben Stiller and Robert De Niro.

In 2009, she starred in the feature film 2:13 and the Hallmark Channel movie Expecting a Miracle with Jason Priestley and Cheech Marin.

[4] She appeared in the 2009 television pilot for Washington Field, a show about the National Capitol Response Squad, a unit of the FBI composed of elite experts in different areas who travel around the world responding to events that concern American interests.

[7] After The Fosters ended its five-season run, Polo continued the role in guest appearances on the spinoff Good Trouble.

[8] In July 2020, the cast and producers of The Fosters united for a virtual table read of the pilot episode to benefit the Actors Fund, to help provide COVID-19 relief.

[9] Polo has guest-starred on many TV shows, including The Practice, Felicity, Chicago Hope, Numb3rs, Sports Night, Frasier, Ghost Whisperer, Medium, Law & Order: LA, Castle and Criminal Minds.

Continuing to blend movies and television in her career, Polo took roles in JL Ranch and its sequel The Wedding Gift, All For Nikki, Deadly Switch, The Ravine, and Fourth Grade.

Her return to television brought Polo full circle to her first love, dance, when she landed the role of former ballet dancer Julia in the Fox series The Big Leap, opposite Scott Foley and Piper Perabo.