The Diocese of Escuintla is a Latin Church ecclesiastical territory or diocese of the Catholic Church in Guatemala.

It is a suffragan diocese in the ecclesiastical province of the metropolitan Archdiocese of Guatemala.

The Diocese of Escuintla was erected on 28 July 1994 from a territorial prelature.

Its cathedral is Catedral Nuestra Señora de Concepción, in the episcopal see of Escuintla.

It was established on 1969.05.09 as a Territorial Prelature of Escuintla on territory split off from the Archdiocese of Guatemala, which remains its metropolitan.