The Pioneers compete as members of the Lone Star Conference in basketball, soccer, softball and volleyball, and as an independent in gymnastics.

The gymnastics team competes in the Midwest Independent Conference which comprises NCAA Division I, II and III institutions.

TWU also fields teams in artistic swimming, dance, stunt, and wrestling.

As an AIAW Division I team in the 1979 Women's College World Series, the Pioneer softball team won the national championship by defeating UCLA, 1–0, in the deciding game, led by pitcher Kathy Arendsen.

[4] In 1973, TWU reached the national championship match of the AIAW women's volleyball tournament, only to fall to Long Beach State.