The Best of Hot Tuna is a Hot Tuna compilation album released in 1998.

It covers songs from all the Hot Tuna albums released on Grunt Records.

Bill Thompson, former manager of Jefferson Airplane and Hot Tuna helped select the songs for inclusion.

The artwork on the cover is a painting of Jorma Kaukonen and Jack Casady made by Grace Slick.

The album was released as a double CD.