The group's line-up consisted of Daniel Huggins Williams (fiddle), John Munnerlyn (banjo), Cloet Hamman (acoustic guitar), Henry Bogan (cello), and an unidentified second guitarist.

Williams, the designated frontman and primary songwriter of the ensemble, was a rare left-handed fiddler who also later tutored other musicians such as Johnny Gimble.

[2] Since the members were by majority not full-time musicians, the group preferred to perform regionally at social events and dance halls.

[3] In addition to the group's progressive arrangements, they also incorporated jazz and cajun influences they encountered while listening to radio programming.

In 1930, Munnerlyn departed the group to move to Houston and was replaced by Shorty Lester, whose brother, Henry, occasionally played fiddle in live performances and the Serenaders' final recording sessions.