The trio are backed by a three-piece band featuring Martin Kolarides on guitar, Henrik Jensen on double bass and Peter Ibbetson on drums.

The group's second album, The Rise and Fall of Ruby Woo, includes original compositions by Puppini, Stephanie O'Brien and Kate Mullins.

The group appeared in the 2009 Jonathan Creek New Year's special The Grinning Man, performing their 2007 single "Spooky" and has been featured on the soundtracks for the US TV series Grey's Anatomy and Chuck.

From March 2016, the trio embarked on a tour to promote the album across many areas in the country and internationally finishing off in London at O2 Islington Academy.

Two versions of the song exist, one being that which was used for the video and the other is a track on The Real Tuesday Weld's 2008 album The London Book of the Dead.

The group used period costumes designed by Vivienne Westwood in their video for "Jilted", an original song written by Marcella Puppini (not to be confused with the 1954 Theresa Brewer country number).

In November 2012, the Sisters featured on the album Electro Swing V by Bart & Baker collaborating with The Real Tuesday Weld on the song "Last Tango in Clerkenwell".

In late 2018, the trio sang with The Real Tuesday Weld on their new song, "Don't Get High No More", and the video was premiered on YouTube on 6 January 2019.