Focused on suicide prevention efforts among lesbian, gay, bisexual, transgender, queer, and questioning (LGBTQ) youth, they offer a toll-free telephone number where confidential assistance is provided by trained counselors.

They discovered that no such helpline existed and decided to dedicate themselves to forming the resource: an organization to promote acceptance of LGBTQ youth, and to aid in crisis and suicide prevention among that group.

[9] While continuing various educational and media campaigns (including a PSA with Daniel Radcliffe)[10] in 2006 a new short documentary was released consisting of interviews with hotline staff telling stories of calls from LGBTQ youth that they had received.

[11] By 2007 the hotline had received over 100,000 calls since its inception, over 3000 education kits had been distributed, and hundreds of questions had been asked through the "Dear Trevor" online forum from around the world.

[9] During National Suicide Prevention Week a digital advertisement campaign named "Don't Erase Your Queer Future" was undertaken which included banner ads on social media websites and The Trevor Project website which encouraged reflection by displaying quotes and progressively erasing words to show how many artistic, cultural, and social contributions would be lost if prominent LGBTQ people would have committed suicide.

[16][17] In 2021, the first openly gay active NFL player, Carl Nassib, used his coming out to also announce a $100,000 donation to The Trevor Project.

"[23][24] The project's Lifeguard Workshop Program uses a structured, age-appropriate curriculum to address topics around sexuality, gender identity, the impacts of language and behavior, and what it means for young people to feel different.