Thermomonosporaceae is a family of bacteria that share similar genotypic and phenotypic characteristics.

The family Thermomonosporaceae includes aerobic, Gram-positive, non-acid-fast, chemo-organotrophic Actinomycetota.

They produce a branched substrate mycelium bearing aerial hyphae that undergo differentiation into single or short chains of arthrospores.

The presence of the diagnostic sugar madurose is variable, but can be found in most species of this family.

The polar lipid profiles are characterized as phospholipid type PI for most species of Thermomonospora, Actinomadura and Spirillospora.