The man drove the two hours to the isolated mansion to find that it was haunted by various creatures after him.

Smith is attacked by a witch, a skeleton, an Indian warrior, an archer, and Frankenstein's monster (Ed Payson).

The third and last in the Pete Smith Audioscopiks 3D series of shorts, Third Dimensional Murder used footage shot specifically for it, unlike the previous two shorts which utilized test footage shot by Jacob Leventhal and Jack Norling.

As with the two previous Audioscopiks short films, the prints were in red-green anaglyph by Technicolor.

This film opens in 2-D color, with a young woman showing how to hold the 3-D viewer.