[2] The zoo's focus changed over the years, and it now houses an impressive animal collection including reindeer, owls, raccoon dogs, parrots, mongooses, otters, snakes, tarantulas, lizards and various invertebrates.

[5] In April 2018, the Americas Zone opened, featuring Brazilian tapir, capybara, macaw, greater rhea, marabou stork, and Jamaican boa.

Tilgate Nature Centre has become nationally important for maintaining breeding groups of these animals to preserve a variety of endangered wild species.

Critically endangered animals include Northern bald ibis, Utila spiny-tailed iguana, Laysan duck and swift parakeet.

[2] In 2020, Tilgate Nature Centre received a Gold Star Award (Switch To A Reusable) for Sustainability and Zoostainability, from the British and Irish Association of Zoos and Aquariums (BIAZA).