Founder and CEO Chris Prynoski began working at MTV in New York on shows such as Daria, Beavis and Butt-Head, and his own creation, Downtown, which was nominated for a primetime Emmy Award.

[8] The California studio later added a wholly owned subsidiary, Robin Redbreast, which was then unionized in order to produce Motorcity for Disney XD.

[10] In April 2016, Titmouse released its first feature-length film, the R-rated Nerdland, which stars Paul Rudd and Patton Oswalt.

[11] Two years later, in 2018, Titmouse became the studio behind the Disney and Lucasfilm YouTube series of one-minute shorts, Star Wars: Galaxy of Adventures.

[14][15][a] In January 2022, the employees of Titmouse New York formed a union with Animation Guild IATSE Local 839, the first to do so outside of Los Angeles County in more than 70 years.

[18] Titmouse and Netflix co-produced the Pendleton Ward and Duncan Trussell-created series The Midnight Gospel, which aired the same year.