The Toledo Subdivision is a railroad line owned and operated by CSX Transportation in the U.S. state of Ohio.

The line runs 167.8 miles (270.0 km) from Hamilton (north of Cincinnati) north to Perrysburg (near Toledo).

[1] It was originally built by predecessors of the Baltimore and Ohio Railroad.

[3][4] Later that decade in 1859, the Dayton and Michigan Railroad opened, continuing the line to Toledo.

[5] The lines passed to the Baltimore and Ohio Railroad and CSX through leases and mergers.