The town is on the Northern Highway and is in the Shire of Campaspe and the City of Greater Bendigo local government areas, 142 kilometres (88 mi) north of the state capital, Melbourne.

[2] During the Victorian Gold Rush and after the discovery of gold at nearby Rushworth, Toolleen was a busy intersection where the roads linking Bendigo, Beechworth, Melbourne and the Murray River port of Echuca crossed.

[3] Toolleen Post Office opened on 12 July 1872.

[4] The town is now mainly an agricultural region, with wine grape growing becoming more popular.

[3] Media related to Toolleen, Victoria at Wikimedia Commons