The show is hosted in live-action segments by Bill, MeTV's "cartoon curator", along with his puppet friend, Toony the Tuna.

Each episode contains four to six animated shorts taken from classic series including Looney Tunes/Merrie Melodies, Tom and Jerry, Betty Boop, Popeye, Color Rhapsody, Woody Woodpecker, and many others.

Toon In with Me harkens back to locally produced children's programs that aired from the 1950s through the 1990s, with a live-action host, comedy and puppet segments in between classic cartoons.

As they present all of the cartoons, Bill and Toony deal with various issues in the studio, video chat with Goldie and receive useful information from game show host Mr. Quizzer (also played by Fleming).

During Christmas seasons, they also showcased the 1948 Rudolph the Red-Nosed Reindeer short by Max Fleischer produced for the Jam Handy Organization, which is also in the public domain.