Totternhoe is a village and civil parish in the Manshead hundred of the county of Bedfordshire, England.

Totternhoe is an ancient village in southern Bedfordshire, near Dunstable and Leighton Buzzard.

Totternhoe Castle, of motte-and-bailey design, was built during the Norman period, probably during the years of the Anarchy, only the mound survives.

Behind the knoll is a large chalk quarry producing Totternhoe Stone and modern lime kilns.

The Domesday Book of 1086 recorded the village as Totene Hou, meaning "look out house" and "spur", presumably describing forts on the Knoll.