It is the largest and longest continuously operating intercity bus carrier in New York State, an interline partner with Megabus, and a member of the National Trailways Network.

[7] During the 1930s, following a series of early successes in the then newly-emerging bus industry, service was first extended from its original Hudson Valley and Metro New York home into the Adirondacks.

During this time service was expanded to include Long Island and the nearby Canadian cities of Montreal, Quebec and Toronto, Ontario.

A long term revenue sharing partnership was also established with Greyhound Bus Lines to provide enhanced service and reliability to the customers of both brands.

[12] Trailways of New York has been a chief “front of shirt” sponsor of the Kingston Stockade Football Club since its inception in 2015[13] and runs special “fan buses” to away games.

The following routes are operated by Adirondack Trailways: New York-Kingston-Albany-Glens Falls-Montreal, Babylon-Kingston, Newburgh-Kingston, Kingston-Saugerties-Oneonta, Binghamton-Oneonta-Albany, Albany-Utica-Syracuse, Albany-Gloversville, Glens Falls-Warrensburg-Massena, and Syracuse-Canton-Massena.

In August 2018, Trailways of New York launched its brand LINE, a “business class” route running between midtown Manhattan, the Hudson Valley, and the Catskills.

As of its launch, LINE stops included West 31st Street in Manhattan and the New York state towns of New Paltz, Kingston, Woodstock, Phoenicia, and Hunter.