Between 1996 and 2004 they competed in the FA Premier League National Division, then the top tier of the English women's football pyramid.

They are currently members of the North West Women's Regional Football League Premier and play their home games at the Ellesmere Port Sports Village.

[6] Tranmere won the next tier of the English women's football pyramid – the FA Premier League Northern Division – at the first attempt, with only one defeat all season.

[13] In 1999, Tranmere won the Reebok Women's Football Festival in Mansfield, then the traditional curtain-raiser to the season, beating local rivals Everton 1–0 in the final.

[12] Though the game was level at half time, Arsenal dominated and eventually ran out 3–0 winners, to secure their fourth successive title.

/> In the 2010–11 season, Tranmere came 12th and last in the Northern Combination,[25] and were relegated to North West Regional League, Premier Division.

[27] Rovers finished 3rd in the North West Regional Football League in the 2023-24 season, behind Blackburn Community Sports Club Women and the champions Cheadle Town F.C.

[34] Upon its completion, the club are due to move their home games to Tranmere's training ground, Solar Campus.

[35][needs update] Note: Flags indicate national team as defined under FIFA eligibility rules; some limited exceptions apply.

[37] Goalkeeper Jo Fletcher also played for England,[38] whilst Katie Williams has represented Wales twenty times.