[1][2][3][4][5] It was inaugurated on 1 May 1784 following the reforms of Lorena that banned the burial of a deceased in the church.

The monumental cemetery, later expanded into the terrain of the ridge of the Terzollina stream, incorporated the Pilastro villa, in the 15th century belonged to the Davanzelli and then passed on to the Tassinari in 1786, and the villa the Almonds, already belonged to the Dominican fathers of San Marco.

Modern access is marked by a row of cypresses marking the monumental architecture of the place of silence, designed by architect Umberto Fabbrini.

In the cemetery of Trespiano are buried the remains of the Carlo and Nello Rosselli, Giuseppe Poggi, Pietro Chesi, Mario Fabiani, Lando Conti, Piero Bargellini, Gaetano Pieraccini, Ernesto Rossi, Gaetano Salvemini, Piero Calamandrei, Spartaco Lavagnini, Aldobrando de 'Medici Tornaquinci, Ettore Nava, Luigi Dallapiccola, Ugo Schiff, Paola Pezzaglia, Carlo Betocchi, Rosa Balistreri, Luigi Michelet, Gillian Brilli Peri, Clement Biondetti, Roberto Assagioli, Mason Remey, and Giaele Covelli.

In Trespiano Florentine cemetery the ashes of Dora d'Istria, aka the Princess Helena Koltsova-Massalskaya, born Elena Gjika are still kept today.