[1] It has been heavily exploited for agricultural use as an effective biopesticide, having been frequently cited as an alternative biological control agent in the regulation of fungi-induced plant diseases.

Fungi in this genus are able to adapt to different ecological niches and can colonize their habitats effectively, allowing them to be powerful antagonists and biocontrol agents.

[5] Typical of Trichoderma species is having a fast growth rate and the production of green or hyaline conidia on a branched conidiophore structure.

This aggregate consists of 12 species within three lineages that have similar morphology as the "true" T. koningii but can be differentiated from each other by their phenotypic characters and geographic distributions.

[1] Some phialides on widely-spaced branches are flask-shaped, resembling a wine bottle, whereas some tend to have a very swollen middle when in dense clusters or "pseudo-whorls".

[3] T. koningii typically produces smooth and ellipsoidal (egg-shaped) conidia, with a mean length of 4.1–4.3 μm, that aggregates in a slimy green mass at the tip of the phialides.

[9] In culture, colonies display rapid growth on potato dextrose agar (PDA), as cream-coloured in the beginning but later turns green because of sporulation.

During conidial production, colouration first begins at the centre then later spreads outward in dark or dull green concentric rings that are vague to noticeable.

This fungus is capable of biosynthesizing silver nanoparticles, volatile organic compounds and secondary metabolites such as trichokonins, koninginins, and pyrones.

Other polyketides reportedly isolated from T. koningii are Trichodermaketones A-D, 7-O-Methylkoninginin D, and 6-pentyl alpha pyrone which can inhibit the germination of other fungal spores.

The extracellular activity occurs through the reaction between the calcium in the environment and oxalic acid secreted by the fungus, leading to the production of biomineral species.

T. koningii also thrives in other environments, including growing on decaying wood, in marine species, estuarine sediments, and in mines and caves.

[18] T. koningii antagonizes S. cepivorum by acting as a secondary colonizer of the infected plant roots and secreting enzymes that cause the degradation and lysis of the pathogen.

Koninginins bear similar structural elements as flavonoids and vitamin E. They can inhibit the process of inflammation caused by snake bites.