They have a negative staining reaction with Melzer's reagent, and feature a conspicuous hilar appendage.

The basidia (spore-bearing cells) are club-shaped with clamps at the base, and measure 24–32 long by 4.8–5.6 μm wide.

[1] The subtropical fungi Tricholosporum atroviolaceum and T. pseudosordidum are similar species with small spores.

They can be distinguished from T. tropicale by their less robust fruit bodies, and microscopically by the relative dearth of cystidia.

[1] The type collection of Tricholosporum tropicale was discovered in September, fruiting singly on the ground in a cacao plantation.