Each subpopulation is isolated and the species mostly occurs in forests (both the interior and at edges), but also in nearby hedges and plantations.

[1][2] The Usambara three-horned chameleon has a sail-like ridge on the back and can reach up to 35 cm (14 in) in total length.

[2] It is typically green overall, often with a paler or darker pattern, and may also show some yellow.

[2] The Usambara three-horned chameleon is generally common in its range and listed as Least Concern (not threatened) by the IUCN.

Between 1992 and 2011 a total of 8,437 live individuals were exported from Tanzania for the pet trade, 622 of which were not captured from the wild, but bred or born in captivity.