The work was commissioned by the Castilian merchant Jean de Sedano, and features the Virgin and child in an enclosed garden.
[1] Specifically, the figures are painted in a manner similar to Jan van Eyck's work, particularly Adam and Eve,[2] while the overall design and composition seem derived from Hans Memling.
[3] In 1503, de Sedano joined the Confraternity of the Holy Blood, a sect devoted to the veneration of a 12th-century relic brought from Jerusalem.
The donor appears kneeling in prayer in the left wing, being blessed by John the Baptist, with his young son by his side.
In the year he joined the Confraternity, de Sedano commissioned David to produce a painting of the Marriage at Cana, now also in the Louvre.