[5] At the next Newmarket meeting thirteen days later Trophonius was one of eleven three-year-olds, from an original entry of twenty-three, to contest the third running of the 2000 Guineas over the Rowley Mile course.
Ridden by Sam Barnard, the black colt started the 5/2 favourite and "won easy"[6] from Lord Grosvenor's filly Barrosa with Magus third and Hit-or-Miss fourth.
[9] On 30 May, Trophonius was moved up in distance to contest the Derby Stakes over one and a half miles at Epsom Downs Racecourse.
He was entered in two races at Newmarket's First October meeting but was withdrawn from both events, with Robert Andrew paying a forfeit on both occasions.
[10] Trophonius remained in training as a four-year-old but failed to win in five races beginning with an unplaced effort behind Rainbow in a sweepstakes at Newmarket on 1 May.
He was withdrawn from the Garden Stakes, finished third to Woful in a handicap race over the Abington Mile and was beaten by Lord Jersey's three-year-old Orson in a five furlong match.