Troups Creek begins in northern Troupsburg, Steuben County, New York.
The creek then turns south and begins flowing parallel to New York State Route 36.
In this township, the creek almost immediately receives the tributary North Brook and begins flowing parallel to Pennsylvania Route 249.
A short distance further downstream, it picks up the tributaries South Brook Troups Creek and Biscuit Hollow.
[3] It is possible to canoe on 6.8 miles (10.9 km) of Troups Creek during a rapid snowmelt or within two days of heavy rain.