Tsukubusuma Shrine (都久夫須麻神社, Tsukubusuma Jinja) is a Shinto shrine on Chikubu Island in Shiga Prefecture, Japan.

Its main hall (本殿, honden) is a National Treasure of Japan.

The shrine's main festival is held annually on June 15.

It is also called Chikubushima Shrine (竹生島神社, Chikubushima Jinja).

This article relating to Shinto is a stub.