[4] She graduated with her PhD from Nagoya University School of Science in 1956,[5] which was also the year that she met her husband, Reiji Okazaki.

[4] Tsuneko and Reiji Okazaki's early research consisted of studying DNA synthesis and specific nucleotide characteristics in frog eggs and sea urchins.

[4] Years later, after much research done in both the U.S and Japan, in 1968, Tsuneko and Reiji published their breakthrough findings on Okazaki fragments in PNAS.

[7] Tsuneko has continued to be involved in different research projects up to this day, mainly investigating different aspects of DNA.

She contributed to research on the human centromere protein B found to induce translational positioning of nucleosomes on α-satellite sequences.

[11] Additionally, throughout the years of 2004 to 2007, her main job was in the Stockholm office, where she was the director of the Japan Society for the Promotion of Science.