Before then, Queens had competed in the South Atlantic Conference (SAC) for 20 of their varsity sports; the men's and women's swimming and diving teams competed in the Bluegrass Mountain Conference and the men's volleyball team competed in the Independent Volleyball Association, a scheduling alliance among schools that are independents in that sport's National Collegiate division.

On May 7, 2022, Queens announced it had accepted an invitation from the ASUN Conference and would join the league on July 1.

In addition to the listed sports, Queens considers its cheerleaders, both male and female, as well as its all-female dance team, to be varsity athletes.

The facility is home to the basketball, swimming & diving, volleyball, and wrestling programs and was built in 2012 to replace the Ovens Athletic Center.

A notable feature of the complex is Rex, the world's largest standing lion statue.