Tuvok's backstory is told during the first episode of Voyager, where he worked as an undercover Federation agent aboard a Maquis ship, the Val Jean, led by Chakotay.

The starship Voyager, under the command of Captain Kathryn Janeway, is sent to extract Tuvok, but the mission is interrupted as both ships are catapulted across the galaxy by a mysterious force.

Janeway leads both crews on a return trip home for seven years in uncharted space, creating the basic setting of the series.

When Jara did not return his affections, Tuvok's jealousy caused him to be expelled from school and resulted in his parents sending him to a Vulcan master to learn emotional control.

Upon graduation, he was commissioned an ensign at age 29, serving as a junior science officer on the USS Excelsior, under Captain Hikaru Sulu as seen in the episode "Flashback".

It is revealed during "Endgame", the final episode of the series, that Tuvok suffers from a degenerative neurological disease, which can only be cured by undergoing a mind meld with a family member.

However, in his elderly years, in an alternate timeline, the disease finally ravaged Tuvok's mind since he was unable to return to the Alpha Quadrant in time for a cure.

To save Tuvok, Captain Janeway's future self changed history by finding a way to return the Voyager to the Alpha Quadrant before his disease worsened.

He is also shown to exhibit subdued moments of annoyance, self-doubt, sarcasm and even anger, mostly directed towards Neelix, whose gregarious personality is often at odds with Tuvok's Vulcan stoicism.

The mirror universe Tuvok appears in the Star Trek: Deep Space Nine third-season episode "Through the Looking Glass" as a member of the Terran Rebellion.

In 2015, Tim Russ again reprised the role of Tuvok in the fan series, Star Trek: Renegades as head of Section 31 alongside Walter Koenig portraying the ST:TOS character of Pavel Chekov.

In the MMORPG video game Star Trek Online, Tim Russ provides voice acting for the character of Tuvok, who plays a major role in the Species 8472 quest line.