Twemlow is divided by the Dane from the adjoining parishes of Holmes Chapel and Brereton to the south.

The parish is also bounded by Goostrey to the north, Lower Withington and Swettenham to the east, and Cranage to the west.

The Dane Valley Way long-distance footpath passes through the parish on its route from Buxton to Northwich.

The viaduct has attracted fans of the singer Harry Styles since it featured in a 2013 film about the rise of One Direction.

In 2024, Network Rail fenced off part of the structure and erected a specially constructed wall with blackboards for fans to inscribe their names, with the aim of preventing further damage after many fans inscribed their names on the structure.