Jim and Jason started the business using bulk milk tanks converted into fermenters that were donated to them by their grandfather who was a retired dairy farmer.

Two Brothers Brewing has now been in business for over 20 years and has opened multiple locations throughout the Chicago metropolitan area, as well as one in Arizona.

[5] Two Brothers Brewing opened first in Warrenville, Illinois in the year 1996 when Jim and Jason Ebel returned to the United States after living in France.

Using their prior experience working in a brewery located in Denver, they went into production using bulk milk tanks that were donated to them from their grandfather.

Two Brothers Tap House remains in Warrenville, Illinois where they offer tours of their newly equipped brewery to the public every weekend.

[7][8] Two Brothers Brewing owners Jim and Jason Ebel then decided to open their first location outside of the state of Illinois in Scottsdale, Arizona.

[11][12] Jim and Jason Ebel set their eyes on opening the Two Brothers Social Tap in Oak Park, Illinois sometime in June, 2017.

[16][17] Extra money was spent purchasing the latest energy-efficient equipment including an ultra-high efficient boiler which allows for the conservation of water during the brewing process.

[17][16] To provide their customers with the most fresh produce, Two Brothers facilities grow their own ingredients in their organic rooftop gardens, as well as purchase their products locally from within 200 miles of the restaurant's locations which reduces their carbon footprint by minimizing transportation distance.

[17] The mission of Green Chicago Restaurant Coalition is to "empower members of the food service industry and dedicated diners alike to reduce our collective footprint, bolstering the health of our planet and its people.

"[19] Two Brothers has also joined the Natural Resources Defense Council to support the use of clean water in brewing practices.

The states in which Two Brothers Brewing products are currently available include Illinois, Connecticut, Florida, Indiana, Minnesota, New York, Ohio, Missouri, Iowa, Pennsylvania, Wisconsin, Arizona, and New Jersey.