[2] Tylosin is used in veterinary medicine to treat bacterial infections in a wide range of species and has a high margin of safety.

[2] Like other macrolides, tylosin has a bacteriostatic effect on susceptible organisms, caused by inhibition of protein synthesis through binding to the 50S subunit of the bacterial ribosome.

[citation needed] Tylosin has a wide spectrum of activity against Gram-positive bacteria including Staphylococcus, Streptococcus, Corynebacterium, and Erysipelothrix.

[3][5] The following represents MIC susceptibility data for a few medically significant pathogens: Tylosin has been used to treat a variety of different diseases throughout the world.

[citation needed] Tylosin may increase digitalis blood levels, thus its toxicity,[5] and may be antagonistic to chloramphenicol or lincosamides.