Biddle departed Newport, Rhode Island, 1 November 1901 for Port Royal, South Carolina where she went into reserve.

Following recommissioning 28 May 1902 she cruised with the Torpedo Boat Flotilla along the Atlantic coast and in the West Indies until January 1903.

Biddle spent the summer cruising with the Atlantic Torpedo Fleet and then went into reserve at Charleston Navy Yard 18 November 1909.

In October 1911 she shifted to the Reserve Flotilla Division at Annapolis, Maryland, and went into ordinary at the Naval Academy, 13 March 1914.

After serving with the Pennsylvania Naval Militia (June–September 1915) Biddle reverted to the Annapolis Reserve torpedo Division.