USS Darlington was a captured Confederate steamer acquired by the Union Navy from the prize court during the American Civil War.

She was put into service by the Union Navy to patrol navigable waterways of the Confederacy to prevent the South from trading with other countries.

Darlington, a Confederate 300 ton steamer, was captured by boats from USS Pawnee in Cumberland Sound, Florida, 3 March 1862, with a cargo of army wagons, ammunition, and camp equipment on board.

She was taken over and, with a crew of 23 and one howitzer as armament, USS Darlington was employed by the Union Navy for use in the waters off Fernandina, Florida, and Port Royal, South Carolina, with Acting Master J. W. Godfrey in command.

This article incorporates text from the public domain Dictionary of American Naval Fighting Ships.