On 6 January Rear Admiral David Dixon Porter assigned Juliet to 1st Division of Light Draft Gunboats where she served the Mississippi Squadron as an escort vessel maintaining communications along the river and protecting the vital flow of shipping which sustained military and naval strength throughout the campaigns and cut the Confederacy in two with the fall of Vicksburg and Port Hudson.

While the Union ships were at Springfield Landing making preparations to clear away the sunken hulk of an old steamer which had stopped their progress toward Shreveport, Louisiana, a messenger arrived with word that General Banks had suffered a severe defeat near Mansfield and was falling back to Grand Ecore.

On 26 and 27 April Juliet again and again fought off cannon and musketry, suffering 16 casualties including 2 killed and heavy damage to the ship.

After repairs at Cairo, Illinois, Juliet recommissioned 6 September and returned to duty with the Mississippi squadron, serving as an escort and patrol vessel.

After the end of the war, Juliet decommissioned at Mound City, Illinois, 30 June 1865, and was sold at public auction there to Philip Wallach 17 August 1865.