USS Port Royal was a double-ended gunboat built for the U.S. Navy during the American Civil War.

The vessel was assigned to patrol the rivers and other waterways of the Confederate States of America and to enforce the Union blockade on the South.

She engaged Confederate batteries at Sewell's Point, Virginia, 8 May and a week later participated in the Battle of Drewry's Bluff, on the James River below the southern capital.

After General Robert E. Lee's seven day campaign turned back McClellan's thrust, Port Royal shifted operations to the North Carolina Sounds.

She was part of the Union Naval force which reconnoitered the Neuse River, North Carolina, arid attacked Kinston, 12–16 December.