Repairs and alterations at the navy yard there took her into August, when she resumed tests and exercises in the Block Island-New London area.

At the end of December, the S-boat proceeded to Staten Island, whence, on 4 January 1924, she headed south to participate in Fleet Problem III, a test of Caribbean Sea defenses and transit facilities of the Panama Canal.

In mid-July 1925, S-50 returned to New London and for the next two years was primarily engaged in conducting experimental tests and performing exercises for the Submarine School.

During that period, she regularly interrupted those duties for annual overhauls and individual, division, and fleet exercises; and, in July 1926, was called on to assist in salvage operations for sister ship USS S-51.

At the end of the month, she proceeded to Philadelphia, Pennsylvania, where she was decommissioned on 20 August 1927 and remained berthed as a unit of the inactive fleet there until the 1930s.